design

Note: I simplified technical terms and details whenever possible to keep this post focused on the design problems and accessible to a larger audience. If you want to stitch more than 2 bits of DNA together, the most popular method these days is probably Golden Gate Assembly. In a nutshell, you mix DNA fragments in a tube where enzymes will cut their flanks to create overhangs (also called sticky ends), then fuse the overhangs together to form the desired DNA construct:...